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Old 05-19-2014, 06:08 AM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238
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If you prepare libraries with DNA samples described there, there will be two downstream issues. Firstly, accurate quantification of libraries for clustering with multiple peaks is difficult and inaccurate quantification can result in over or under clustering which will affect read number or quality or both, although that might be considered sequencing service provider problem. Secondly, small fragments are more efficient in cluster formation in comparison with large fragments and more of your reads will be from sequencing smaller fragments.
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