Why 50
I don't think 50 is the number to shift. In Jason's paper, they did "all reads aligning to the + strand were offset by +4 bp, and all reads aligning to the – strand were offset −5 bp". In fact, whether you shift or not, the distribution of reads will not significantly change.
Originally posted by blakeoft
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I don't think 50 is the number to shift. In Jason's paper, they did "all reads aligning to the + strand were offset by +4 bp, and all reads aligning to the – strand were offset −5 bp". In fact, whether you shift or not, the distribution of reads will not significantly change.
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