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Old 03-19-2014, 07:42 AM   #3
Location: Livermore, CA

Join Date: Apr 2011
Posts: 27

We find that the primary cause of concatamers (assuming the RNA is abundant and of high quality) is over amplification in the final PCR step. I would try decreasing the number of PCR cycles. Alternatively, if you have low quality or very small amounts of RNA you should decrease the primer concentration during cDNA synthesis.
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