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Old 01-18-2016, 01:34 AM   #1
wisnu.wicaksono
Junior Member
 
Location: New Zealand

Join Date: Jan 2016
Posts: 3
Default Why do we need index sequencing primer

Hi all,

just wondering why do we use index sequencing primer and why the sequencing primer (forward or reverse) can not be used to read the whole sequence including the barcode/index. Based on illumina video ( https://www.youtube.com/watch?v=womKfikWlxM), the sequencing primer only read just before the barcode). Thank you and have a nice day.
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