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Old 04-08-2020, 02:36 PM   #3
Location: Livermore, CA

Join Date: Jan 2016
Posts: 44

You can also try a 95C denaturation (instead of the usual NaOH denaturation). 1 minute at that temperature (in RSB or EB should be fine), just make sure you have a decent volume there so you don't evaporate everything. TruSeq Custom Amplicon product does this method to make the libraries nice and single stranded for cluster generation.
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