I'm a complete newbie to bioinformatics and have just started analyzing my RNA-seq data (illumina HiSeq2000 100 bp reads) and have hit a snag. I'm trying to run the fastx_quality_stats and I keep getting this error message:
fastx_quality_stats: failed to open input file '/home/vallejov/stevia_R1.fastq': Value too large for defined data type
I get the same message if I try to run fastx_trimmer. Does this mean that my file is too large? my file is 48G. Do I need to parse it? If I parse it, when do I need to merge the output files again?
Veronica
fastx_quality_stats: failed to open input file '/home/vallejov/stevia_R1.fastq': Value too large for defined data type
I get the same message if I try to run fastx_trimmer. Does this mean that my file is too large? my file is 48G. Do I need to parse it? If I parse it, when do I need to merge the output files again?
Veronica
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