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  • #1

    Best/ cheapest Platform for RNA-Seq

    I am a MS student working with soil microbiome. I am looking for the presence of a gene family in soils under certain conditions and would like to use RNASeq to profile its expression in soil community. Gene is conserved in bacteria and fungi, so I will be analyzing the transcriptomes of both. Illumina HiSeq is off the table, but Im looking into Illumina MiSeq, IonTorrent, or Roche 454. Anyone have any insight?
    Thanks
    Tags: illumina miseq, rnaseq library, soil microbiome
  • #2
    If you need volume sequencing with the lowest error rate then of the two remaining (454 is deprecated) MiSeq is the clear winner. If you don't have access to a local core then you can comparison shop at allseq.com or genohub.com.

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