Hi all,
What kind of difference is expected when quantifying dsDNA on a nanodrop vs. Qubit?
Our core facility has generated Qubit values for my cDNA sampels that are consistently 1.3- to 2.3-fold higher than the Nanodrop values that I generated. I've read a lot of reports claiming that Nanodrop consistently overestimates concentration, so I'm a little puzzled by these Qubit results.
Any help is greatly appreciated!
Thanks in advance,
Amy
What kind of difference is expected when quantifying dsDNA on a nanodrop vs. Qubit?
Our core facility has generated Qubit values for my cDNA sampels that are consistently 1.3- to 2.3-fold higher than the Nanodrop values that I generated. I've read a lot of reports claiming that Nanodrop consistently overestimates concentration, so I'm a little puzzled by these Qubit results.
Any help is greatly appreciated!
Thanks in advance,
Amy
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