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Originally posted by GenoMax
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IMHO this is not necessary; Lane X should look good, that's more important :-) We do this from time to time with chipseq data. -
If there was a control lane defined then there should be no need to go messing about with matrix or phasing settings (assuming the control lane looked OK).Originally posted by victorsor View Post
If you've still got no clusters then the only remaining remedy would be to run the bclToFastq with the --no-eamss option to see if the qualities temporarily dip and then recover.Comment
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Thank you all. We will try to do your suggestions when run finish. I hope that not all data go to waste...Comment
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...Yesterday, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM
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