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Old 12-11-2013, 07:44 AM   #3
Location: Sao Paulo/Brazil

Join Date: Aug 2012
Posts: 22
Default Yes

Yes, I reinstalled and reformated the database several times.
Using the nt database downloaded from ncbi, it works perfectly. Formating my own database based on a genome I have, if worked perfectly too, but just in the first time. This is the strangest thing: It worked once before.

I installed the blast2 from repositories.
I have a genome in *.fasta.

The database was formated with this command:
formatdb -p F -i My_genome.fasta

Was generated the files My_genome.fasta.nhr, .nin and .nsq.
The analysis was run with this command:
blast2 -p blastn -i MY_FILE.fq -d My_genome.fasta -o MY_FILE.blast.out -a 32
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