Does anyone have a workflow or knows of a kit that allows for whole transcript single cell RNA Seq with reverse transcriptase barcoding of each cDNA, and also UMI labeling? We are currently interested to perform this in our lab but most vendors are cutting down to 5' or 3' ends for scRNA-Seq analysis and we still want the whole transcript.
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Originally posted by dwong View PostNuGEN has a kit that has all the abovementioned features, the Ovation SoLo RNA-Seq Kit.
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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