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Old 08-22-2016, 11:59 AM   #1
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Join Date: Jan 2013
Posts: 9
Default 16S-V4 library peak at smaller bp in bioanalyzer trace

Dear All,

I used 16S-V4 kit(BIOO Scientific) to make libraries of DNA extracted from fresh plasma. But the dominant peak is at 350bp, instead of 450bp as suggested by the kit protocol. It's my first time to use this kit. Would anyone kindly help me what this peak could be and what I should do to make it correct?

BTW: the input DNAs were all below 5ng, so I strictly follow the protocol the manufacturer provided and set the PCR II cycles at 22.

Thanks much!

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