It seems our MiSeq mate paired data isn't orientated as 5'--3 3'---5'
In a sequence that would be
5'-------------3'
We see
5'----- 3'-----
Additionally each FASTQ file is mixed with both mate pair ends.
Is there a tool which will reverse one of these fastq files to get
5'----- -----3'
??
Thanks,
J
In a sequence that would be
5'-------------3'
We see
5'----- 3'-----
Additionally each FASTQ file is mixed with both mate pair ends.
Is there a tool which will reverse one of these fastq files to get
5'----- -----3'
??
Thanks,
J
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