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Old 10-10-2017, 07:39 AM   #4
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,235

In Nextera protocol tagmented DNA PCR reaction contains index primers/adapters and PPC. Index primers bind to overhangs from tagmentation oligo and fragments with full length adapters is synthesised. Index primers quantity is not enough for whole amplification cycles so PPC oligos bind to 5' end of adapter or library fragments for further amplification. PPC sequences are short and complementary to 5' end of index adapters so it reduces the cost.
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