View Single Post
Old 02-19-2013, 08:07 AM   #3
GenoMax
Senior Member
 
Location: East Coast USA

Join Date: Feb 2008
Posts: 7,091
Default

MiSeq has known problems with libraries that have low nucleotide diversity. There are several threads (an example thread: http://seqanswers.com/forums/showthread.php?t=27563) on SeqAnswers that address that issue. There are workarounds proposed but no definite solution as yet.

As for the answers regarding the chemistry used for the 3 platform and the differences search through these FAQ's.
GenoMax is offline   Reply With Quote