Hello everyone,
I am trying to create reverse complement of a bacteria gene in minus strand. Though I have read a lot on its mechanism and even got a perl script done that generates reverse complement of a sequence in Fasta format.
But, I need to generate it for bases in rows, with separate columns of genome position, reference, mutation, reference supporting reads and variant supporting read. Will appreciate suggestion regarding suitable language for it.
Regards,
Seema
I am trying to create reverse complement of a bacteria gene in minus strand. Though I have read a lot on its mechanism and even got a perl script done that generates reverse complement of a sequence in Fasta format.
But, I need to generate it for bases in rows, with separate columns of genome position, reference, mutation, reference supporting reads and variant supporting read. Will appreciate suggestion regarding suitable language for it.
Regards,
Seema
Comment