Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Counting of SNPs in different genomic regions

    Hi,
    After annotating the SNP.vcf files using SnpEff v 4.2, I would like to count how many SNPs are found in different genomic regions such as upstream, gene, exon, intron, CDS, 5' UTR, 3' UTR, downstream and intergenic. To perform the SNPs count, I am looking for a program or perl- or python script for few days in different forums. I have annotated SNP.VCF and gene.txt file obtained from SNpEff. Can anyone help me - how can I count the number of SNPs found in different genomic regions? I would highly appreciate your help. Thanks

  • #2
    I've forgotten how SnpEff outputs information, but in general using 'grep -c' is a quick (but mostly manual) way.

    If you want to count lots of things at once, isolate out the categories (e.g. using 'cut'), then run 'sort' and 'uniq -c'. Alternatively, import the data into R and use 'table'.

    Comment


    • #3
      Thanks a lot Gringer. I used SnpSift Extract Fields to get the effect of SNPs in different regions. Then the number of SNPs in different regions are counted in Excel. Finally I got SNPs number in different genomic regions.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin


        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
        Yesterday, 07:01 AM
      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      55 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      51 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      45 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      55 views
      0 likes
      Last Post seqadmin  
      Working...
      X