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Old 09-27-2013, 12:54 PM   #1
mkalive73
Junior Member
 
Location: Arizona

Join Date: Sep 2013
Posts: 1
Default using NEB kit to make libraries for Illumina MiSeq

Hello,

I used NEB multiplex kit to make libraries for Illumina MiSeq. Trying to figure out the amount of sample to load in the flow cell to get a decent cluster density. Last round I loaded 12pM of total sample and it over clustered with my coverage very low. My colleague uses 7pM of the same kind of sample on a HiSeq, how does that compare with MiSeq? should Miseq sample amount be lesser or higher?

Thanks for your suggestions.
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