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Old 05-07-2015, 06:04 PM   #1
DNATECH
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Location: Davis, CA

Join Date: Mar 2015
Posts: 29
Default HiSeq3000/4000 duplicate removal

Hi All,

Our Illumina FAS mentioned that in underloaded lanes on the patterned flow-cells even PCR-free libraries can generate significant numbers of duplicates (clusters swapping over the "walls" into the next empty nanowell if given enough time?).
http://dnatech.genomecenter.ucdavis....data-download/
Would you know of any software that can analyze/remove duplicates based on the coordinate information in the read headers (without any alignments)?

Thanks in advance,
Lutz

Last edited by DNATECH; 05-07-2015 at 07:01 PM.
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