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Old 02-28-2012, 08:52 AM   #2
Location: Ireland

Join Date: Sep 2011
Posts: 86

Unfortunately, I'd say that very high Tween 20 would probably interfere with DNA binding to the flow cell. I don't think anyone would have done any definitive studies on this.

On the bright side, wasting thousands of dollars/euros/lives via minor errors is part and parcel of next-gen sequencing. Happens to us all at some point or another.
Rocketknight is offline   Reply With Quote