Hello. Looking for some help. We are using solexa to sequence a large pool of PCR products (Vent). Vent doesn't not produce overhangs. Therefore to ligate solexa adapters we will have to either enzymatically add the A overhangs or we would use adapters designed to be blunted directly to our PCR product. My question is: Are the A overhangs essential for efficient adapter ligation? Why do they use the overhangs in the first place?
Thanks many-
John
Thanks many-
John
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