SEQanswers - View Single Post - ExoSap purified PCR product compatible for Illumina sequencing?

wingtec · 10-30-2014, 11:55 AM

Thanks, CMBETT.

For me a bigger concern is wether this will mess up the denaturation step, especially in the case where the ExoSap reaction does not get high enough dilution based on quantitation. Quantitation after the purification is also a concern, because of the introduction of the proteins.

Best,

Wing

10-30-2014, 11:55 AM	#3
wingtec Member Location: Charlottesville, VA Join Date: Apr 2010 Posts: 34	Thanks, CMBETT. For me a bigger concern is wether this will mess up the denaturation step, especially in the case where the ExoSap reaction does not get high enough dilution based on quantitation. Quantitation after the purification is also a concern, because of the introduction of the proteins. Best, Wing