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Old 10-23-2017, 12:56 AM   #2
David Eccles (gringer)
Location: Wellington, New Zealand

Join Date: May 2011
Posts: 838

It is a type of technical replicate, but will have some degree of biological variation from the different samples. Usually "technical replicate" refers to an additional sequencing library prepared from the same extract.

In this case because there is a chance of biological variation, I would keep the samples separate and add the sample as an additional factor in any statistical models.
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