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Old 04-25-2018, 04:09 AM   #2
nucacidhunter
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Location: Melbourne

Join Date: Jan 2013
Posts: 1,171
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There are commercially available unique duel indices from venders such as Illumina, NEB, Bioo Scientific, NuGEN (up to 96 UDI) and Integrated DNA technologies (up to 384). These are offered as adapters (except NEB) and you could use them to design unique duel index primer set for your application. The advantage of using these is that they have been validated and less likely to cause any issue.

TruSeq kits based on ligating duel indexed adapters (either combinatorial or unique) do not use indexed primers.
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