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Old 03-16-2017, 06:56 AM   #3
Senior Member
Location: CT

Join Date: Apr 2015
Posts: 242

The end of v3 is ~515. V1-V3 should be using pairs like 27f & 519R. 27F, 173F are both forward primers and won't give you any product? I'm guessing you were given these sequences from someone else? I'd go back and clarify what primers they actually used.
Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
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