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Old 02-23-2018, 01:38 PM   #11
Senior Member
Location: CT

Join Date: Apr 2015
Posts: 237

Both of those look very underclustered. No where near 500k. One tiny thing that can ruin your cluster density is old NaOH. When was the last time you've made 2N from 10N stock? You're diluting the 2N fresh each time right?
Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
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