Hi all,
I want to use the PippinPrep for size selection of my TruSeq small RNA libraries. In the TruSeq kit they give the specs for selection an a polyacrylamide gel. They however compensate (according to Illumina) for a ~7 nt deviation in size from what is expected for adapters adding 118 nt. According to them a 21 nt insert fragment will run at ~146 on a PAA gel. Is there somebody who has successfully used the PippinPrep (3% cassettes) and will be able so tell me with the settings they used to select the fragment containing the 21 and 24 nt inserts?
Thanks
Marike
I want to use the PippinPrep for size selection of my TruSeq small RNA libraries. In the TruSeq kit they give the specs for selection an a polyacrylamide gel. They however compensate (according to Illumina) for a ~7 nt deviation in size from what is expected for adapters adding 118 nt. According to them a 21 nt insert fragment will run at ~146 on a PAA gel. Is there somebody who has successfully used the PippinPrep (3% cassettes) and will be able so tell me with the settings they used to select the fragment containing the 21 and 24 nt inserts?
Thanks
Marike
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