Originally posted by chadn737
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When you do the column purification to remove the DNase enzyme, do you add buffers (which buffer/what volume?) to your RNA sample before passing it though the column and proceed with the same protocol you used initially to extract the RNA? (With Qiagen Mini kit: RLT, RW1, RPE.) I need to remove some DNA from my RNA samples and I'm a bit concerned that you said the inactivation agent isn't completely removed. I want to make sure the cDNA created from my RNA libraries isn't automatically digested. Thanks for your input.
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