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Old 01-09-2012, 02:54 PM   #3
PeteH
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Location: Melbourne

Join Date: Jun 2010
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Quote:
Originally Posted by simonandrews View Post
These sorts of pattern are pretty common (almost to the point of being universal) in RNA-Seq libraries. Apparently it's the 'random' hexamer priming which isn't as random as you might hope.
I think Simon may be referring to this paper:
Hansen, K.D., Brenner, S.E. & Dudoit, S. Biases in Illumina transcriptome sequencing caused by random hexamer priming. Nucleic Acids Res 38, e131e131 (2010) (http://www.ncbi.nlm.nih.gov/pubmed/20395217)
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