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Old 02-07-2013, 09:02 AM   #8
Senior Member
Location: Boston,MA

Join Date: Nov 2008
Posts: 121

We use the Kappa enzyme mix with our own primers and use PhiX as a standard curve.

The main reason we don't use the Quant Kit itself is it is far more expensive than buying the enzyme and primers seperately. That being said, we are a core so we are generally QCing 20 or more samples at a time so the standard curve is but a small component of the cost.
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