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Old 02-07-2013, 10:03 PM   #10
Location: Pennsylvania

Join Date: Sep 2010
Posts: 20

Check Illumina's qPCR protocol. The primer sequences are listed in there.

We are also using the Kapa master mix together with our own primers and PhiX as standard for qPCR. While we are also getting fairly consistent results, our qPCRs tend to overestimate the concentration of our libraries, especially for RNA-seq libraries. Has anyone else seen this? We also tried the Kapa primers and standards but got similar results.
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