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Old 11-20-2009, 07:35 AM   #18
454Sequencing
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Location: Branford, CT

Join Date: Apr 2009
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Hi Phillip,

One clarification - the Rapid Library protocol actually employs a single adaptor moiety, and no longer an A-B pair of adaptors. This is why there is no more ssDNA melt step and why there are no worries of the different adaptor-library combinations you mentioned. Additionally, the RL Adaptor is fluorescently labeled which enables direct quantitation following library synthesis. Your local Roche rep should have access to some material on this topic that would be of assistance.

Best regards,
Jason
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