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Old 02-25-2014, 03:35 AM   #2
Location: Iceland

Join Date: Apr 2012
Posts: 28

Hi Federico,
have you confirmed that the data you have is indeed strand specific?
You can use the script from the RSeQC package to check it out -

Depending on the library preparation protocol you either use the '-s reverse' or '-s yes' option. Do you know the specifics of the library prep? In any case, you will find out if you manage to run ''.

blanco is offline   Reply With Quote