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Old 02-27-2019, 04:34 AM   #1
Adiro
Junior Member
 
Location: Israel

Join Date: Jan 2019
Posts: 3
Default ERCC spike-ins for prokaryotes

Hi!
I see that many people use ERCC spike-ins with prokaryotes, but looking at the manufacturer description I see that they only mention that it is good for eukaryotes... https://www.thermofisher.com/order/c...roduct/4456740

I found some articles that asses the validity of the ERCC in eukaryotes, but never on prokaryotes...

So can I use it in prokaryotes? anyone found an article assessing the validity of ERCC in prokaryotes?


**I'm specifically interested in e.coli, and I really need spike ins because I suspect that most of the genes will express differently between the two conditions... If for example all the genes will be 2 times more expressed in condition A than in condition B, without spike-ins the differential expression will look like there was no change. If you have other suggestions for this scenario besides ERCC, I would love to know... **
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