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Old 03-31-2009, 02:52 AM   #1
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Location: Barcelona

Join Date: Mar 2009
Posts: 3
Default cDNA synthesis kits with titanium

I want to sequence ESTs from a RNA pool. I heard good things about SMART cDNA synthesis kits from Clotech and Evrogen. Also, I read different papers that have used this technology. My concern it is that I also read from "cDNA Frequently Asked Questions" from Roche, that SMART kits are not recommended with titanium kits because the size of the cDNA fragments obtained, ussually small in size and fail to nebulize properly. Any suggestions?
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