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Old 03-31-2009, 05:32 AM   #2
kmcarr
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Location: USA, Midwest

Join Date: May 2008
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Could you post a link to that FAQ. I searched the web site but could not find it. I'm interested in what they mean by the cDNA fragments are usually small in size.

We have run many, many cDNA projects on the 454 using GS 20, GS-FLX and now Titanium. The vast majority of these used SMART cDNA preparation, some with the Evrogen normalization, some without. We have had generally good results. Compared to genomic DNA sequencing cDNA sequencing generally produces fewer filter passed reads (more failures due to mixed reads) and the mean length is shorter. There is also more variability from library to library which probably is a DNA quality issue. I'd be happy to provide more information if you like.
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