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Old 04-17-2009, 12:23 AM   #6
Location: UK

Join Date: Oct 2008
Posts: 19

Hi Gerald

Are you doing a size exclusion with the cDNA and a titration (concentrate the titration at the lower end)? Also, because fragment size is usually shorter for cDNA it may be worth loading the beads on the PTP at a lower density. As I've said before don't expect cDNA runs to be as successful as runs using gDNA.
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