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Old 05-20-2009, 04:17 AM   #8
Emanuel Heitlinger
Location: Karlsruhe

Join Date: Mar 2009
Posts: 11

Hi all,
the Evrogen MINT kit worked better than the SMART kit for me. The SMART kit produced strange concatenated primer/adaptor artefacts. Currently it is used without any polyT modifications producing ~75.000 good quality sequences of ~250nt from 1/4 plate GS FLX run.

Only problem: High amount of rRNA contamination ~50% in the best preparations.
Does anybody else have this problem using SMART or MINT kits?

I'm looking forward to get my first titanium runs...
Emanuel Heitlinger is offline   Reply With Quote