Hi all,
we just got our GS Junior and I am currently designing primers for amplicon sequencing. Does anyone have some experience with it? For example which amplicon size is acceptable for biderectional sequencing? Is there a cut of in primer length an GC content? What is the maximum acceptable size difference for amplicons in emPCR not affecting the coverage? I know that Roche gives some parameters but im really interested to know what will work in real life.
Thank You all!
we just got our GS Junior and I am currently designing primers for amplicon sequencing. Does anyone have some experience with it? For example which amplicon size is acceptable for biderectional sequencing? Is there a cut of in primer length an GC content? What is the maximum acceptable size difference for amplicons in emPCR not affecting the coverage? I know that Roche gives some parameters but im really interested to know what will work in real life.
Thank You all!
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