Hi there, I am a complete RNA seq newbie, so let me excuse myself preventively for any stupid questions.
Recently I started working with mouse RNA seq data, after running the data though Tophat2 I got a 91.0% overall read alignment rate and a 83.1% concordant pair alignment rate.
Could somebody with more experience in the field tell me if this are good alignment values, and if not which are the normal values I should expect ?
Thanks in advanced.
Recently I started working with mouse RNA seq data, after running the data though Tophat2 I got a 91.0% overall read alignment rate and a 83.1% concordant pair alignment rate.
Could somebody with more experience in the field tell me if this are good alignment values, and if not which are the normal values I should expect ?
Thanks in advanced.
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