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Old 03-02-2012, 03:29 AM   #9
Location: San Diego

Join Date: Nov 2011
Posts: 25

Hello Colin

Thanks. That link points to the webpage of the MaxSeq. The miniSeq is another product. It still uses SBS chemistry, but the automation level is totally different. Its called the mini basically because the throughput is lower than the MaxSeq.
About the read lenght: the MaxSeq can read 100bp fragments as well. Its just a matter of using 2 55bp kits. Nevertheless, the throughput is already much higher than 100GB per run.
The polonator was a product developed entirely at the Church lab, at Harvard. It used sequencing by ligation and only polonies for library construction. The MaxSeq uses some of the ideas from the polonator (hardware part only) but has been optimized to work with Sequencing by synthesis and to allow both polony and DNA nanoballs library construction methods. The mini is a totally different instrument at the automation level, so it has little to do with the polonator.
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