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Old 03-28-2013, 10:09 AM   #1
Gina_P
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Location: New York

Join Date: Dec 2011
Posts: 20
Default Beads sticking to tube during Ribo-Zero protocol

Hi all. I noticed that the Ribo-Zero magnetic beads tend to stick to the sides of the tube after you add your RNA sample and mix with pipetting and vortexing, and that this effect carries on through the end. When I finally pipette the 90 uL of sample from the magnetic stand, I usually just am very careful to guide the tip to the liquid without touching any sides since there will be beads stuck to it. However, recently I did the protocol and the sides of the tubes looked much cleaner than normal. My only thoughts are that I used a different brand of PCR tube during the incubation with the removal solution... any other ideas for why this may have changed? Or what your experiences are using Ribo-Zero?
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