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Old 02-05-2020, 01:46 PM   #1
smsestes
Junior Member
 
Location: RTP, NC, USA

Join Date: Feb 2020
Posts: 2
Question bioanalyzer and qubit disagreement

We prepare clinical samples with a formal SOP and do not regularly see irregularities. Today we have a capture pool of samples that quanted at 24.0ng/uL on qubit. To determine fragment size we also run sample pool in duplicate on bioanalyzer DNA1000. Duplicate 1, there is noise around 40bp (<1ng/uL) and no peak in the expected range (300-400). Duplicate 2, there is noise around 40bp and a wide peak between 700 and 1500 (upper marker). We reran the sample pool (4 replicates) on the bioanalyzer with only the noise around 40bp.

Qubit concentration = normal
bioanalyzer fragment size = missing?

Any ideas what is going on? Where is the missing peak?

Last edited by smsestes; 02-05-2020 at 01:47 PM. Reason: clarity
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