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Old 05-16-2016, 10:38 PM   #3
AnushaC
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Location: San Diego

Join Date: Sep 2013
Posts: 78
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Hi,

I did read the link you mentioned which gives an example of cross dimer

iii) Cross Dimer: Primer cross dimers are formed by intermolecular interaction between sense and antisense primers, where they are homologous. Optimally a 3' end cross dimer with a ΔG of -5 kcal/mol and an internal cross dimer with a ΔG of -6 kcal/mol is tolerated generally.
Cross Dimer

But gives no explanation of cross homology , nor any example justifying why it is a cross-homologous primer
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