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Old 03-04-2009, 07:07 AM   #2
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Join Date: Mar 2009
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My understanding is that they use a standard A-tailing. The genomic fragments have 3'-A tails. The adaptors have a 5'-T overhang, not a 3'. This way you should get slightly improved ligation due to the 1-bp annealing in the ligation rxn. The overhangs also will prevent concatemers from forming.
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