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Old 06-15-2018, 06:31 AM   #1
davalbuq
Junior Member
 
Location: Portugal

Join Date: Jun 2018
Posts: 5
Default Problem with the library DNA quantity and quality

Hi all,

It is totally new on the NGS world. Today, I started to prepare my first library preparation by using SureSelect Agilent technology. I did 6 samples (for now) and when I check the quantity/quality of my pre-capture by using the Bioanalyzer 1000 DNA chip I was not totally happy with the results. Effectively, all my 6 curves are more likely with the bad one (I attached an example). So, I understand that I have now several fragments around 300-700 pb. I was wondering about what could be happened? What I did wrong and if I can continue the protocol?

Thank you for your help and any tips will be well received regarding the library preparation

Regards,
David
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