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Old 06-15-2018, 06:55 AM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,186
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It seems that shearing has not resulted in expected fragment length so library size distribution is too large for SureSelect. Average library insert should be around 150 bp.

It is better to prepare another batch of libraries and not to continue with this one. You can check sheared DNA length distribution before proceeding with library prep.
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