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Old 04-18-2017, 05:16 AM   #460
Kristian
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Location: Europe

Join Date: May 2015
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Hi Brian,

Quote:
Originally Posted by Brian Bushnell View Post
Is this a Nextera long-mate pair library?
This is Nextera XT prep, and the adapters/linker were trimmed along with demultplexing directly through Illumina RTA pipeline. On my end the bbduk2 left and right trimming had almost no effect, and the splitnextera also had only a few hits (0.1%) to the adapter, so I'd guess the dataset is (for the most part) trimmed.

Last edited by Kristian; 04-18-2017 at 05:21 AM.
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