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Old 11-23-2010, 04:37 AM   #1
CatVincent
Junior Member
 
Location: Germany

Join Date: Nov 2010
Posts: 2
Default Cluster density Illumina

Hello, I would like to intoduce myself.
We have been preparing and successfully sequencing a number of transcriptome libraries for PE Illumina sequencing.

We obtained unexpectedly high cluster density with (supposedly) only 5nanoM of a transcriptome library loaded on a flow cell.
The library was prepared using a NEB mRNA sample preparation kit and appeared normal when checked on a Bioanalyzer. <
Has anyone else obtained (too) high cluster density with low sample input?

Any comments welcome.
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