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Old 03-06-2013, 06:39 PM   #4
weigrc
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Location: Hong Kong

Join Date: Oct 2011
Posts: 46
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We prepare libraries by ourselves. With the same one kits (TruSeq DNA + Exome kits), we did see the duplicate rate increased between 1st and 2nd batch of library preparation. The cDNA libraries prepared by TruSeq RNA kit may have the same issue since both DNA/RNA are using the same enzymes.

Wei
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